Journal: International Journal of Molecular Sciences

Article Title: Arhgap29 Deficiency Directly Leads to Systemic and Craniofacial Skeletal Abnormalities

doi: 10.3390/ijms26104647

Figure Lengend Snippet: Experimental results of osteogenesis in mandibular tissue. ( A , B ) Von Kossa staining of mandibular tissue sections from E17.5 mice. ( C ) ALP staining of mandibular tissue sections from E17.5 WT mice. ALP-positive cells (shown in blue) are distributed on the surface of the bone matrix. ( D ) Immunohistochemical staining results of mandibular tissue sections from E17.5 WT mice. Arhgap29 -positive cells (shown in brown-yellow) are located on the surface of the bone matrix. (The red arrow indicates osteoblasts).

Article Snippet: Subsequently, the cells were stained for 30 min with either an ALP staining solution (Solarbio, Beijing, China) or an alizarin red S staining solution (OriCell, Guangzhou, China) at pH 8.3.

Techniques: Staining, Immunohistochemical staining

Journal: International Journal of Molecular Sciences

Article Title: Arhgap29 Deficiency Directly Leads to Systemic and Craniofacial Skeletal Abnormalities

doi: 10.3390/ijms26104647

Figure Lengend Snippet: In vitro cell experiment results. ( A ) qPCR assay for osteoblast markers in cells 3 days after they were induced to differentiate. ( B , C ) ALP staining of WT and si Arhgap29 cells 7 days after they were induced to differentiate. ( D , E ) ARS staining of WT and si Arhgap29 cells after they were induced to differentiate for 14 days. ( F ) Quantitative analysis of alkaline phosphatase staining in cells ( n = 5). ( G ) Quantitative analysis of alizarin red staining in cells ( n = 5). All data: *** p < 0.001.

Article Snippet: Subsequently, the cells were stained for 30 min with either an ALP staining solution (Solarbio, Beijing, China) or an alizarin red S staining solution (OriCell, Guangzhou, China) at pH 8.3.

Techniques: In Vitro, Staining